/
/
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Title
- 01:15Murine Bronchoalveolar Lavage Fluid (BALF) Collection and Preparation
- 02:21Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid
- 04:56Extracellular Vesicle Quantification
- 05:35Detection of BALF-derived Extracellular Vesicles
- 07:16Results: UFC Method Demonstrates Higher Total Particle Counts and Protein Recover
- 08:25Conclusion
Here, we describe two extracellular vesicle isolation protocols, ultrafiltration centrifugation and ultracentrifugation with density gradient centrifugation, to isolate extracellular vesicles from murine bronchoalveolar lavage fluid samples. The extracellular vesicles derived from murine bronchoalveolar lavage fluid by both methods are quantified and characterized.
