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利用超滤离心技术分离小鼠支气管肺泡灌洗液中的细胞外囊
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
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利用超滤离心技术分离小鼠支气管肺泡灌洗液中的细胞外囊

9,173 Views

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09:18 min

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November 09, 2018

DOI:

10.3791/58310-v

DOI:
10.3791/58310-v

•

09:18 min
November 09, 2018

•

9 Views
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1Department of Medicine, Division of Pulmonary and Critical Care, Women’s Guild Lung Institute,Cedars-Sinai Medical Center, 2Department of Biomedical Sciences,Cedars-Sinai Medical Center, 3Department of Medicine, Smidt Heart Institute,Cedars-Sinai Medical Center

Summary

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在这里, 我们描述了两个细胞外囊泡分离方案, 超滤离心和超离心与密度梯度离心, 从小鼠支气管肺泡灌洗液样品中分离细胞外囊泡。用两种方法对小鼠支气管肺泡灌洗液中的细胞外囊泡进行了定量和表征。

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Parimon, T., Garrett III, N. E., Chen, P., Antes, T. J. Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique. J. Vis. Exp. (141), e58310, doi:10.3791/58310 (2018).

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