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用微计算机断层扫描法对无节段3D 组织学进行固定染色、全尺寸、毫米度试样的刚性嵌入
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07:41 min
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October 17, 2018
DOI:
1The Jake Gittlen Laboratories for Cancer Research,Penn State College of Medicine, 2Division of Experimental Pathology, Department of Pathology,Penn State College of Medicine, 3Medical Scientist Training Program,Penn State College of Medicine, 4Center for In Vivo Microscopy,Duke University Medical Center, 5KTM Research
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我们开发了协议, 并设计了一个定制装置, 以实现毫米级试样的嵌入。我们提出了样品制备程序, 重点是在丙烯酸树脂和聚酰亚胺管中嵌入, 以实现刚性固定化和长期储存标本, 用于审讯组织结构和细胞形态学的显微 CT。
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Lin, A. Y., Ding, Y., Vanselow, D. J., Katz, S. R., Yakovlev, M. A., Clark, D. P., Mandrell, D., Copper, J. E., van Rossum, D. B., Cheng, K. C. Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography. J. Vis. Exp. (140), e58293, doi:10.3791/58293 (2018).
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