Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
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October 17, 2018
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We developed protocols and designed a custom apparatus to enable embedding of millimeter-scale specimens. We present sample preparation procedures with an emphasis on embedding in acrylic resin and polyimide tubing to achieve rigid immobilization and long-term storage of specimens for the interrogation of tissue architecture and cell morphology by micro-CT.
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Lin, A. Y., Ding, Y., Vanselow, D. J., Katz, S. R., Yakovlev, M. A., Clark, D. P., Mandrell, D., Copper, J. E., van Rossum, D. B., Cheng, K. C. Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography. J. Vis. Exp. (140), e58293, doi:10.3791/58293 (2018).
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