00:32Modification of a Baculovirus Expression Vector with either the GP67 Signal Peptideor the Insect Hemolin Signal Peptidefor Plant Protein Secretion Expression
02:24Production and Amplification of Baculovirus Constructs Harboring the Recombinant Protein Expression Cassette
07:02Results: Analysis of Plant Proteins Secreted by Insect Cells
Vi præsenterer her, protokoller for at udnytte den insekt celle og baculovirus protein udtryk system til at producere store mængder af planten udskilles protein til protein krystallisering. Et baculovirus udtryk vektor er blevet ændret med enten GP67 eller insekt hemolin signal peptid for plante protein sekretion udtryk i insekt celler.