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Generatie van Knock-out primaire en uitgebreide menselijke NK-cellen met behulp van Cas9 Ribonucleoproteins
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JoVE 신문 면역학 및 감염병학
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
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Generatie van Knock-out primaire en uitgebreide menselijke NK-cellen met behulp van Cas9 Ribonucleoproteins

12,174 Views

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07:20 min

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June 14, 2018

DOI:

10.3791/58237-v

DOI:
10.3791/58237-v

•

07:20 min
June 14, 2018

•

5 Views
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1Center for Childhood Cancer and Blood Disease,Nationwide Children’s Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine,University of Oxford, 3Center for Clinical and Translational Research,Nationwide Children’s Hospital Research Institute

Summary

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Hier presenteren we een protocol om genetisch wijzigen van primaire of uitgebreide menselijke natural killer (NK) cellen met behulp van Cas9 Ribonucleoproteins (Cas9/RNPs). Met behulp van dit protocol, we genereerden menselijke NK cellen tekort voor Transformeren groeifactor-b receptor 2 (TGFBR2) en hypoxanthine phosphoribosyltransferase 1 (HPRT1).

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Naeimi Kararoudi, M., Dolatshad, H., Trikha, P., Hussain, S. A., Elmas, E., Foltz, J. A., Moseman, J. E., Thakkar, A., Nakkula, R. J., Lamb, M., Chakravarti, N., McLaughlin, K. J., Lee, D. A. Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins. J. Vis. Exp. (136), e58237, doi:10.3791/58237 (2018).

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