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Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

DOI:
10.3791/58237-v

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07:20 min

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June 14, 2018

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, , , , , , , , , , , ,
1Center for Childhood Cancer and Blood Disease,Nationwide Children’s Hospital, 2Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine,University of Oxford, 3Center for Clinical and Translational Research,Nationwide Children’s Hospital Research Institute

Bölümler

  • 00:04Başlık
  • 00:44Human Primary and Expanded Natural Killer (NK) Cell Transduction
  • 04:48Results: Representative Electroporation Efficiency
  • 06:13Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we present a protocol to genetically modify primary or expanded human natural killer (NK) cells using Cas9 Ribonucleoproteins (Cas9/RNPs). By using this protocol, we generated human NK cells deficient for transforming growth factor–b receptor 2 (TGFBR2) and hypoxanthine phosphoribosyltransferase 1 (HPRT1).

Etiketler

Cas9 RibonucleoproteinsNK CellsGene KnockoutPrimary NK CellsExpanded NK CellsCRISPR RNATracrRNAElectroporationTransductionImmunotherapySolid TumorsBrain TumorsTGF-beta

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