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Immobilisation covalente de protéines pour la spectroscopie de Force seule molécule

JoVE 신문
생화학

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JoVE 신문 생화학

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

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Immobilisation covalente de protéines pour la spectroscopie de Force seule molécule

10,574 Views

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11:13 min

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August 20, 2018

DOI:

10.3791/58167-v

DOI:

10.3791/58167-v

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11:13 min

August 20, 2018

•

2 Views

Tanja D. Becke^2,3, Stefan Ness, Stefanie Sudhop³, Hermann E. Gaub, Markus Hilleringmann, Arndt F. Schilling*⁴, Hauke Clausen-Schaumann*^1,3

¹Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, ²FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, ³Center for Nano Science,Ludwig-Maximilians-Universität München, ⁴Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen

Tags

Covalent Immobilization Protein Single Molecule Force Spectroscopy Atomic Force Microscope Cantilever Probe Biomolecule Immobilization Silanization EDC NHS Phosphate-buffered Saline

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