/
/
An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells
Chapters
- 00:04Title
- 00:46Mouse Bone Marrow (BM) and Secondary Lymphoid Tissue Cell Isolation
- 03:40In Vivo Activation, Proliferation, and Th1 Cell Differentiation Assay
- 06:48Results: Representative BM-derived Dentritic Cell-induced Naïve CD4+ T Cell Activation, Proliferation, and Th1 Differentiation
- 07:39Conclusion
Here, we present a protocol for the in vivo determination of naïve CD4 T cell (T cell) activation, proliferation, and Th1 differentiation induced by GM-CSF bone marrow (BM)-derived dendritic cells (DCs). In addition, this protocol describes BM and T-cell isolation, DC generation, and DC and T-cell adoptive transfer.
