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A High-throughput, High-content, Liquid-based C. elegans Pathosystem
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JoVE 신문 면역학 및 감염병학
A High-throughput, High-content, Liquid-based C. elegans Pathosystem

A High-throughput, High-content, Liquid-based C. elegans Pathosystem

DOI:
10.3791/58068-v

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09:44 min

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July 01, 2018

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, ,
1Department of Biosciences,Rice University

Chapters

  • 00:04Title
  • 00:25Preparation and Maintenance of P. aeruginosa and RNAi Bacteria
  • 02:34Maintenance and Preparation of C. elegans
  • 03:21Liquid Killing Assay Setup
  • 05:56Adaptation for Screening Multiple C. elegans Strains or Knockdowns
  • 07:06Results: C. elegans-P. aeruginosa Liquid Killing Pathosystem
  • 08:37Conclusion

Summary

자동 번역

자동 번역

Here we describe a protocol that is an adaptable, whole host, high-content screening tool that can be utilized to study host-pathogen interactions and be used for drug discovery.

Tags

High-throughputHigh-contentLiquid-basedC. ElegansPathosystemHost-pathogen InteractionDrug DiscoveryVirulence FactorsSmall MoleculesP. AeruginosaLB AgarLB BrothSlow Kill (SK) MediaRNAiCarbenicillinIPTGL1 WormsOP50Temperature-sensitiveCell Scraper

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