JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
ホット スポット領域の変異の包括的かつ同時検出のための単一液滴デジタル ポリメラーゼの連鎖反応
1Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department,Institut Curie, PSL Research University, 2CNRS UMR144,Institut Curie, PSL Research University, 3Department of Medical Oncology,Institut Curie, PSL Research University, 4University Versailles Saint-Quentin-en-Yvelines, 5University Paris Descartes, 6Inserm U830,Institut Curie, PSL Research University
Chapters
- 00:04Title
- 00:48Blood Collection and Plasma Storage
- 02:04cfDNA Extraction
- 04:45ddPCR Mix Preparation
- 05:46Droplet Generation
- 06:25PCR Amplification
- 07:06Results: KRAS and EGFR Drop-off ddPCR Assays
- 07:50Conclusion
