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Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
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08:23 min
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September 25, 2018
DOI:
1Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department,Institut Curie, PSL Research University, 2CNRS UMR144,Institut Curie, PSL Research University, 3Department of Medical Oncology,Institut Curie, PSL Research University, 4University Versailles Saint-Quentin-en-Yvelines, 5University Paris Descartes, 6Inserm U830,Institut Curie, PSL Research University
Summary
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Here, we present a protocol to accurately quantify multiple genetic alterations of a target region in a single reaction using drop-off ddPCR and a unique pair of hydrolysis probes.
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Decraene, C., Bortolini Silveira, A., Michel, M., Bidard, F., Pierga, J., Stern, M., Proudhon, C. Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions. J. Vis. Exp. (139), e58051, doi:10.3791/58051 (2018).
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