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CRISPR Guide RNA kloning for pattedyr systemer
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06:48 min
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October 02, 2018
DOI:
1Wyss Institute for Biologically Inspired Engineering,Harvard University, 2Department of Genetics,Harvard Medical School, 3Department of Pathology,Massachusetts General Hospital, 4Institute for Medical Engineering & Science,Massachusetts Institute of Technology, 5Synthetic Biology Center,Massachusetts Institute of Technology, 6Department of Biological Engineering,Massachusetts Institute of Technology, 7Broad Institute
Summary
Automatically generated
Her, er en enkel, effektiv og kostnadseffektiv metode for sgRNA kloning skissert.
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Cite this Article
Nageshwaran, S., Chavez, A., Cher Yeo, N., Guo, X., Lance-Byrne, A., Tung, A., Collins, J. J., Church, G. M. CRISPR Guide RNA Cloning for Mammalian Systems. J. Vis. Exp. (140), e57998, doi:10.3791/57998 (2018).
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