This content is Free Access.
CRISPR Guide RNA Cloning for Mammalian Systems
1Wyss Institute for Biologically Inspired Engineering,Harvard University, 2Department of Genetics,Harvard Medical School, 3Department of Pathology,Massachusetts General Hospital, 4Institute for Medical Engineering & Science,Massachusetts Institute of Technology, 5Synthetic Biology Center,Massachusetts Institute of Technology, 6Department of Biological Engineering,Massachusetts Institute of Technology, 7Broad Institute
Chapters
- 00:04Title
- 00:25Preparation of the Primers and Expression Vectors
- 02:05Ligating the Annealed gRNA Oligonucleotides
- 02:59Transformation of E. coli
- 04:03PCR Analysis
- 05:35Results: CRISPR Guided RNA Cloning
- 06:23Conclusion
