This content is Free Access.
Isolamento, caracterização e alto Throughput análise de fluxo extracelular de Mouse primária Renal Tubular as células epiteliais
16,491 Views
•
09:40 min
•
June 20, 2018
DOI:
1Department of Molecular and Cellular Pharmacology,University of Miami Leonard M. Miller School of Medicine, 2Interdisciplinary Stem Cell Institute,University of Miami Leonard M. Miller School of Medicine, 3Department of Medicine, Division of Cardiology,University of Miami Leonard M. Miller School of Medicine, 4Vascular Biology Institute,University of Miami Leonard M. Miller School of Medicine, 5Peggy and Harold Katz Family Drug Discovery Center,University of Miami Leonard M. Miller School of Medicine
Summary
Automatically generated
Este protocolo fornece uma abordagem econômica para isolar e caracterizar pilhas do rato primária renal tubular que podem posteriormente ser sub cultivadas para avaliar funções biológicas renal ex vivo, incluindo bioenergética mitocondrial.
Explore More Videos
Read Article
Cite this Article
Ding, W., Yousefi, K., Shehadeh, L. A. Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells. J. Vis. Exp. (136), e57718, doi:10.3791/57718 (2018).
Copy