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Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses
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Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses
Chapters
- 00:05Title
- 00:54C. albicans Preparation
- 02:29Deep Dermal Injection
- 03:57Sample Collection and Preparation for Histological Staining and Slides
- 05:09Hematoxin & Eosin Staining
- 06:07Periodic Acid-Schiff Staining
- 07:19Sample Collection and Preparation for RNA Extraction
- 08:30Results: Histological Analysis of C. albicans Infection in Mice
- 10:00Conclusion
Here we describe a protocol that allows histological and molecular analysis of skin samples after Candida albicans intradermal injection. This protocol maintains the structural integrity of the skin and allows for the localization of tissue-resident or newly recruited immune cells as well as the pathogen distribution.
