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Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses
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Immunology and Infection
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JoVE Journal Immunology and Infection
Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses

Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses

English

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10,523 Views

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10:45 min

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June 13, 2018

DOI:

10.3791/57574-v

DOI:
10.3791/57574-v

•

10:45 min
June 13, 2018

•

16 Views
, , , ,
1Department of Biotechnology and Biosciences,University of Milano-Bicocca, 2Harvard Medical School and Division of Gastroenterology,Boston Children’s Hospital

Summary

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Here we describe a protocol that allows histological and molecular analysis of skin samples after Candida albicans intradermal injection. This protocol maintains the structural integrity of the skin and allows for the localization of tissue-resident or newly recruited immune cells as well as the pathogen distribution.

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Santus, W., Mingozzi, F., Vai, M., Granucci, F., Zanoni, I. Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses. J. Vis. Exp. (136), e57574, doi:10.3791/57574 (2018).

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