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Maken, testen en het gebruik van kalium-Ion selectieve Microelectrodes in weefsel plakjes van volwassen hersenen
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Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain
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Maken, testen en het gebruik van kalium-Ion selectieve Microelectrodes in weefsel plakjes van volwassen hersenen

11,764 Views

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11:20 min

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May 07, 2018

DOI:

10.3791/57511-v

DOI:
10.3791/57511-v

•

11:20 min
May 07, 2018

•

8 Views
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1Department of Physiology, David Geffen School of Medicine,University of California Los Angeles, 2Department of Neurology, David Geffen School of Medicine,University of California Los Angeles, 3Department of Neurobiology, David Geffen School of Medicine,University of California Los Angeles

Summary

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Kalium ionen bijdragen tot de rustpotentiaal membraan van cellen en extracellulaire K+ concentratie is een cruciale regulator van cellulaire prikkelbaarheid. We beschrijven hoe te maken, te kalibreren en te gebruiken monopolaire K+-selectieve microelectrodes. Deze elektroden, kunt de meting van elektrisch evoked K+ concentratie dynamiek in volwassen hippocampal plakjes.

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Cite this Article

Octeau, J. C., Faas, G., Mody, I., Khakh, B. S. Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain. J. Vis. Exp. (135), e57511, doi:10.3791/57511 (2018).

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