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Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain
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JoVE Journal 神経科学
Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

11,778 Views

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11:20 min

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May 07, 2018

DOI:

10.3791/57511-v

DOI:
10.3791/57511-v

•

11:20 min
May 07, 2018

•

8 Views
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1Department of Physiology, David Geffen School of Medicine,University of California Los Angeles, 2Department of Neurology, David Geffen School of Medicine,University of California Los Angeles, 3Department of Neurobiology, David Geffen School of Medicine,University of California Los Angeles

概要

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Potassium ions contribute to the resting membrane potential of cells and extracellular K+ concentration is a crucial regulator of cellular excitability. We describe how to make, calibrate and use monopolar K+-selective microelectrodes. Using such electrodes enables the measurement of electrically evoked K+ concentration dynamics in adult hippocampal slices.

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Cite this Article

Octeau, J. C., Faas, G., Mody, I., Khakh, B. S. Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain. J. Vis. Exp. (135), e57511, doi:10.3791/57511 (2018).

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