Ammonia fiber expansion (AFEX) is a thermochemical pretreatment technology that can convert lignocellulosic biomass (e.g., corn stover, rice straw, and sugarcane bagasse) into a highly digestible feedstock for both biofuels and animal feed applications. Here, we describe a laboratory-scale method for conducting AFEX pretreatment on lignocellulosic biomass.
Chundawat, S. P. S., Pal, R. K., Zhao, C., Campbell, T., Teymouri, F., Videto, J., Nielson, C., Wieferich, B., Sousa, L., Dale, B. E., Balan, V., Chipkar, S., Aguado, J., Burke, E., Ong, R. G. Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass. J. Vis. Exp. (158), e57488, doi:10.3791/57488 (2020).