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Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites
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Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites
Chapters
- 00:04Title
- 01:08Bone Marrow-derived Macrophage (BMM) Isolation and Differentiation
- 04:05BMM Coverslip Plating
- 05:22L. amazonensis Infection and Parasite Quantification
- 07:21Results: Representative BMM Infection Analyses
- 09:06Conclusion
All pathogenic Leishmania species reside and replicate inside macrophages of their vertebrate hosts. Here, we present a protocol to infect murine bone marrow-derived macrophages in culture with Leishmania, followed by precise quantification of intracellular growth kinetics. This method is useful for studying individual factors influencing host-pathogen interaction and Leishmania virulence.
