ジャーナル
/
免疫学と感染
/
Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

DOI:
10.3791/57486-v

•

10:01 min

•

March 16, 2018

•

, , , ,
1Department of Cell Biology and Molecular Genetics,University of Maryland

章

  • 00:04標題
  • 01:08Bone Marrow-derived Macrophage (BMM) Isolation and Differentiation
  • 04:05BMM Coverslip Plating
  • 05:22L. amazonensis Infection and Parasite Quantification
  • 07:21Results: Representative BMM Infection Analyses
  • 09:06Conclusion

概要

自動翻訳

自動翻訳

All pathogenic Leishmania species reside and replicate inside macrophages of their vertebrate hosts. Here, we present a protocol to infect murine bone marrow-derived macrophages in culture with Leishmania, followed by precise quantification of intracellular growth kinetics. This method is useful for studying individual factors influencing host-pathogen interaction and Leishmania virulence.

タグ

MacrophageLeishmaniaVirulenceIntracellular GrowthBone Marrow-derivedIn VitroMurineParasite QuantificationTherapyLeishmaniasisMutated StrainsTherapeutic CompoundsIsolationDissection

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

ScanLag: High-throughput Quantification of Colony Growth and Lag Time

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

Read Article