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The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

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The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

DOI:

10.3791/57469-v

•

09:47 min

•

May 25, 2018

•

Hiroyuki Yamamoto, Yoshikazu Sawaguchi, Michio Kimura

¹Department of Microbiology and Molecular Cell Biology,Nihon Pharmaceutical University, ²Department of Clinical Pharmaceutics,Nihon Pharmaceutical University

Chapters

00:05Title
00:36Preparation of Tissue Extracts
01:40Digestion of Substrates in Model Protease and Tissue Extracts
02:59Purification of the Iminobiotin-labeled Substrate
05:17Sample Preparation and Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF Mass Spectrometry)
06:48Results: Identification of Protease Specificity
08:46Conclusion

Summary

자동 번역

Here, we present a protocol to determine protease specificity in crude mouse tissue extracts using MALDI-TOF spectrometry.

Tags

Protease Specificity MALDI-TOF Mass Spectrometry PH Manipulation Protease Assay Biotin Labeling Streptavidin Beads

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