Journal
/
Bioquímica
/
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

DOI:
10.3791/57469-v

•

09:47 min

•

May 25, 2018

•

, ,
1Department of Microbiology and Molecular Cell Biology,Nihon Pharmaceutical University, 2Department of Clinical Pharmaceutics,Nihon Pharmaceutical University

Capítulos

  • 00:05Título
  • 00:36Preparation of Tissue Extracts
  • 01:40Digestion of Substrates in Model Protease and Tissue Extracts
  • 02:59Purification of the Iminobiotin-labeled Substrate
  • 05:17Sample Preparation and Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF Mass Spectrometry)
  • 06:48Results: Identification of Protease Specificity
  • 08:46Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we present a protocol to determine protease specificity in crude mouse tissue extracts using MALDI-TOF spectrometry.

Tags

Protease SpecificityMALDI-TOF Mass SpectrometryPH ManipulationProtease AssayBiotin LabelingStreptavidin Beads

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/57469?article_type=v

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
check_url/es/57469?article_type=v

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
check_url/es/57469?article_type=v

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
check_url/es/57469?article_type=v

Analysis of Histone Antibody Specificity with Peptide Microarrays
check_url/es/57469?article_type=v

Defining Hsp33’s Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
check_url/es/57469?article_type=v

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
check_url/es/57469?article_type=v

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
check_url/es/57469?article_type=v

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
check_url/es/57469?article_type=v

Nitropeptide Profiling and Identification Illustrated by Angiotensin II
check_url/es/57469?article_type=v

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Read Article