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The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
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Biochemie
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JoVE Journal Biochemie
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

6,689 Views

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09:47 min

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May 25, 2018

DOI:

10.3791/57469-v

DOI:
10.3791/57469-v

•

09:47 min
May 25, 2018

•

1 Views
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1Department of Microbiology and Molecular Cell Biology,Nihon Pharmaceutical University, 2Department of Clinical Pharmaceutics,Nihon Pharmaceutical University

Summary

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Here, we present a protocol to determine protease specificity in crude mouse tissue extracts using MALDI-TOF spectrometry.

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Protease SpecificityMALDI-TOF Mass SpectrometryPH ManipulationProtease AssayBiotin LabelingStreptavidin Beads

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Yamamoto, H., Sawaguchi, Y., Kimura, M. The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes. J. Vis. Exp. (135), e57469, doi:10.3791/57469 (2018).

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