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透射电镜的小型化样品制备方法
JoVE 신문
생물학
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JoVE 신문 생물학
Miniaturized Sample Preparation for Transmission Electron Microscopy
DOI:

09:04 min

July 27, 2018

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Chapters

  • 00:04Title
  • 00:46Cryo Grid Preparation
  • 02:42Grid Preparation Macro
  • 03:56Single-cell Lysate Preparation
  • 05:54Cell Lysis Macro
  • 07:32Results: Imaging of Membrane Structures Using the Cryowriter
  • 08:32Conclusion

Summary

자동 번역

介绍了一种用于透射电镜制备 nanoliter 大小试样体积的仪器和方法。没有纸印迹步骤是必需的, 从而避免了这可能对蛋白质的有害后果, 大大减少样本损失, 并使分析单细胞裂解的视觉蛋白质组学。

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