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Formaldehyd-assisteret Isolation af regulerende elementer til foranstaltning kromatin tilgængelighed i pattedyrceller
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Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

Formaldehyd-assisteret Isolation af regulerende elementer til foranstaltning kromatin tilgængelighed i pattedyrceller

10,910 Views

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08:08 min

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April 02, 2018

DOI:

10.3791/57272-v

DOI:
10.3791/57272-v

•

08:08 min
April 02, 2018

•

11 Views
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1Department of Oncohematology and Genetics. Institute of Biomedicine of Seville (IBiS),University Hospital Virgen del Rocío, 2Institute of Biochemistry, Medical Faculty, Friedrichstrasse 24, Member of the German Center for Lung Research,Justus-Liebig-University

Summary

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Plasticitet af nukleare arkitektur styres af dynamiske epigenetiske mekanismer, herunder ikke-kodende RNA'er, DNA methylering, nucleosome repositionering samt Histon sammensætning og modifikation. Her beskriver vi en Formaldehyde-Assisted Isolation af regulerende elementer (FAIRE) protokol, som giver bestemmelsen af kromatin tilgængelighed i en reproducerbar, billig og ligetil måde.

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Chromatin AccessibilityFormaldehyde-assisted IsolationRegulatory ElementsMammalian CellsDNA-protein Cross-linkingChromatin RemodelingFAIRESonicationChromatin Fragmentation

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Cite this Article

Rodríguez-Gil, A., Riedlinger, T., Ritter, O., Saul, V. V., Schmitz, M. L. Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells. J. Vis. Exp. (134), e57272, doi:10.3791/57272 (2018).

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