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Genetics

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JoVE Journal Genetics
Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
 

Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

Article DOI: 10.3791/57272-v • 08:08 min • April 2nd, 2018
April 2nd, 2018 •
1, 2, 2, 2, 2
1Department of Oncohematology and Genetics. Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, 2Institute of Biochemistry, Medical Faculty, Friedrichstrasse 24, Member of the German Center for Lung Research, Justus-Liebig-University

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요약
Automatic Translation

The plasticity of nuclear architecture is controlled by dynamic epigenetic mechanisms including non-coding RNAs, DNA methylation, nucleosome repositioning as well as histone composition and modification. Here we describe a Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) protocol which allows the determination of chromatin accessibility in a reproducible, inexpensive, and straightforward manner.

Tags

Formaldehyde-assisted Isolation Regulatory Elements Chromatin Accessibility Mammalian Cells DNA Cross-linking Purification Quantification DNA Cleavage Antibodies Sonicator Protocol Growth Medium Formaldehyde Concentration Glycine Quenching Formaldehyde PBS Washing
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