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甲醛辅助分离测定哺乳动物细胞染色质可及性的调控元素
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Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
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甲醛辅助分离测定哺乳动物细胞染色质可及性的调控元素

10,910 Views

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08:08 min

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April 02, 2018

DOI:

10.3791/57272-v

DOI:
10.3791/57272-v

•

08:08 min
April 02, 2018

•

11 Views
, , , ,
1Department of Oncohematology and Genetics. Institute of Biomedicine of Seville (IBiS),University Hospital Virgen del Rocío, 2Institute of Biochemistry, Medical Faculty, Friedrichstrasse 24, Member of the German Center for Lung Research,Justus-Liebig-University

Summary

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核结构的可塑性受动态后生机制的控制, 包括非编码 rna、DNA 甲基化、核重新定位以及组蛋白组成和修饰。在这里, 我们描述了甲醛辅助的调控元素 (自由放任) 协议的隔离, 它允许以可重现、廉价和直截了当的方式确定染色质的可访问性。

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Chromatin AccessibilityFormaldehyde-assisted IsolationRegulatory ElementsMammalian CellsDNA-protein Cross-linkingChromatin RemodelingFAIRESonicationChromatin Fragmentation

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Rodríguez-Gil, A., Riedlinger, T., Ritter, O., Saul, V. V., Schmitz, M. L. Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells. J. Vis. Exp. (134), e57272, doi:10.3791/57272 (2018).

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