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High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method
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High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method

DOI:
10.3791/57241-v

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07:51 min

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May 21, 2018

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,
1Microbiology and Immunology Division, Department of Pathology,University of Utah

Chapters

  • 00:04Title
  • 00:49Identifying Synergy Prediction Mutants from Chemical-genetics Dataset by the Overlap2 Method
  • 01:17Validation of Predicted Synergistic Interactions
  • 04:52High-throughput Screen with Synergy Prediction Mutants to Identify the Novel Synergistic Pairs
  • 05:21Results: Use of Checkerboard Assay to Identify and Validate Synergistic Drug Molecules
  • 07:05Conclusion

Summary

자동 번역

자동 번역

Synergistic drug combinations are difficult and time-consuming to identify empirically. Here, we describe a method for identifying and validating synergistic small molecules.

Tags

Synergistic Drug CombinationsHigh-throughput ScreeningAntimicrobial DrugsInfectious DiseasesE. ColiM9 Minimal MediumSmall MoleculesOptical DensityCheckerboard Assay

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