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Genetics

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JoVE Journal Genetics
Een Optogenetic methode te controleren en analyseren van gen expressiepatronen in cel-naar-cel interacties
 
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Een Optogenetic methode te controleren en analyseren van gen expressiepatronen in cel-naar-cel interacties

Article DOI: 10.3791/57149-v • 07:59 min • March 22nd, 2018
March 22nd, 2018 •
1,2, 1,3,4,5
1Institute for Frontier Life and Medical Sciences, Kyoto University, 2Japan Science and Technology Agency, PRESTO, 3Institute for Integrated Cell-Material Sciences, Kyoto University, 4Graduate School of Medicine, Kyoto University, 5Graduate School of Biostudies, Kyoto University

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Hier presenteren we een protocol te analyseren van cel naar cel overdracht van oscillerende informatie door optogenetic control en live monitoring van genexpressie. Deze aanpak biedt een uniek platform om te testen een functionele betekenis van dynamische gen expressie programma's in meercellige systemen.

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Genetica kwestie 133 optogenetic methode real-time beeldvorming luciferase verslaggever GAVPO oscillerende expressie Notch signalering
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