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Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
1Center for Free Electron Laser Science,DESY, 2Department of Physics,University of Hamburg, 3Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation,University of Hamburg, 4The Hamburg Center for Ultrafast Imaging,University of Hamburg, 5Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), 6European Molecular Biology Laboratory, EMBL c/o DESY, 7Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry
Chapters
- 00:04Title
- 00:38Polydimethylsiloxane (PDMS) Mold Fabrication and In Situ X-ray Chip Fabrication
- 04:59Fluid Delivery Access Ports and Surface Treatment
- 06:51Protein Preparation and Crystallization
- 08:51Results: Crystallization and In Situ X-ray Diffraction of Protein Crystals
- 10:41Conclusion
This protocol describes in detail how to fabricate and operate microfluidic devices for X-ray diffraction data collection at room temperature. Additionally, it describes how to monitor protein crystallization by dynamic light scattering and how to process and analyze obtained diffraction data.
