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基于复用等温放大诊断平台检测 Zika、基孔肯亚和登革热1

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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

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基于复用等温放大诊断平台检测 Zika、基孔肯亚和登革热1

13,927 Views

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06:18 min

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March 13, 2018

DOI:

10.3791/57051-v

DOI:

10.3791/57051-v

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06:18 min

March 13, 2018

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13 Views

Ozlem Yaren¹, Barry W. Alto², Kevin M. Bradley³, Patricia Moussatche³, Lyudmyla Glushakova³, Steven A. Benner^1,3

¹Foundation for Applied Molecular Evolution (FfAME), ²Florida Medical Entomology Laboratory,University of Florida, ³Firebird Biomolecular Sciences LLC

Summary

目前用于检测 Zika、基孔肯亚和登革热病毒的复用诊断程序需要复杂的样本准备和昂贵的仪器, 而且在低资源环境下很难使用。我们显示一个诊断, 使用等温放大与目标特定的链不可替代探针, 以检测和区分这些病毒具有高灵敏度和特异性。

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