/
/
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
14,007 Views
•
06:18 min
•
March 13, 2018
DOI:
Summary
Automatically generated
Current multiplexed diagnostics to detect Zika, chikungunya, and dengue viruses require complex sample preparation and expensive instrumentation, and are difficult to use in low resource environments. We show a diagnostic that uses isothermal amplification with target-specific strand displaceable probes to detect and differentiate these viruses with high sensitivity and specificity.
Read Article
Cite this Article
Yaren, O., Alto, B. W., Bradley, K. M., Moussatche, P., Glushakova, L., Benner, S. A. Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1. J. Vis. Exp. (133), e57051, doi:10.3791/57051 (2018).
Copy