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High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices
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신경과학
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JoVE 신문 신경과학
High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices

6,835 Views

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09:11 min

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March 26, 2018

DOI:

10.3791/56923-v

DOI:
10.3791/56923-v

•

09:11 min
March 26, 2018

•

3 Views
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1Laboratoire Physico-Chimie Curie, Institut Curie, Institut Pierre-Gilles de Gennes pour la microfluidique,Université PSL, CNRS, 2UMR 144 Institut Curie,Université PSL, CNRS

Summary

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Volume is an important parameter regarding physiological and pathological characteristics of cells. We describe a fluorescent exclusion method allowing full-field measurement of in vitro neuronal volume with sub-micrometric axial resolution required for the analysis of neurites and dynamic structures implied in neuronal growth.

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Cite this Article

Braïni, C., Mottolese, A., Ferrante, I., Monnier, S., Villard, C. High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices. J. Vis. Exp. (133), e56923, doi:10.3791/56923 (2018).

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