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Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
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Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
Chapters
- 00:05Title
- 00:44Bone Marrow Macrophage Derivation and Activation with Antibodies: In Vitro Testing
- 05:34Harvesting Peritoneal Macrophages after an IVIg + LPS Challenge: In Vitro Testing
- 08:38Results: BMDM Reaction to IVIg + LPS Treatment
- 10:06Conclusion
Antibody-based drugs have revolutionized treatment for inflammatory diseases. In addition to having direct effects on specific targets, antibodies can activate macrophages to become anti-inflammatory. This protocol describes how anti-inflammatory macrophage activation can be assessed in vitro, using mouse bone marrow macrophages, and in vivo, using peritoneal macrophages.
