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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

7,328 Views

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10:09 min

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January 26, 2018

DOI:

10.3791/56631-v

DOI:
10.3791/56631-v

•

10:09 min
January 26, 2018

•

2 Views
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1Institute for Anatomy and Cell Biology, Department of Molecular Embryology, Faculty of Medicine,University of Freiburg, 2Faculty of Biology,University of Freiburg

Summary

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We present an effective and reproducible method to isolate and culture neural progenitor cells from embryonic and postnatal brain tissue for chromatin immunoprecipitation (ChIP) of histone 3 lysine 79 dimethylation (H3K79me2) - a histone mark located within the globular domain of histone 3.

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Neural ProgenitorsChromatin ImmunoprecipitationHistone 3 Lysine 79 DimethylationCerebral CortexCerebellumBrain DevelopmentEpigenetic ModificationsIn VivoHistone ModificationsCell CultureCerebellar CellsAstrocytesPoly-D-lysine

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Cite this Article

Bovio, P., Roidl, D., Heidrich, S., Vogel, T., Franz, H. Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark. J. Vis. Exp. (131), e56631, doi:10.3791/56631 (2018).

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