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Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds
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April 02, 2018
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This protocol describes a platform for fabricating self-assembled tissue rings in variable sizes using a customized 3D-printed plastic mold. PDMS negatives are cured in the 3D-printed mold; then agarose is cast in the cured PDMS negatives. Cells are seeded into the resulting agarose wells where they aggregate into tissue rings.
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Strobel, H. A., Calamari, E. L., Alphonse, B., Hookway, T. A., Rolle, M. W. Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds. J. Vis. Exp. (134), e56618, doi:10.3791/56618 (2018).
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