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Обнаружение и удаление нуклеиназы загрязнения во время очистки рекомбинантных прототип пенистый вирус интеграза
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JoVE 신문 면역학 및 감염병학
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
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Обнаружение и удаление нуклеиназы загрязнения во время очистки рекомбинантных прототип пенистый вирус интеграза

6,879 Views

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07:50 min

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December 08, 2017

DOI:

10.3791/56605-v

DOI:
10.3791/56605-v

•

07:50 min
December 08, 2017

•

7 Views
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1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

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Nuclease ContaminationProtein PurificationRecombinant Prototype Foamy Virus IntegraseE. ColiSonicationUltracentrifugationFPLCNickel-charged ResinSDS-PAGEUV Absorbance

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Lopez Jr., M. A., Mackler, R. M., Altman, M. P., Yoder, K. E. Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase. J. Vis. Exp. (130), e56605, doi:10.3791/56605 (2017).

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