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Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
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JoVE Journal İmmünoloji ve Enfeksiyon
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

DOI:
10.3791/56605-v

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07:50 min

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December 08, 2017

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, , ,
1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Bölümler

  • 00:05Başlık
  • 00:35Nickel Affinity Chromatography
  • 03:30Heparin Affinity Chromatography
  • 04:28Nuclease Assay
  • 06:46Results: Nuclease Assay of Heparin Sepharose Fractions
  • 07:19Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Recombinant prototype foamy virus integrase protein is often contaminated with a bacterial nuclease during purification. This method identifies nuclease contamination and removes it from the final preparation of the enzyme.

Etiketler

Nuclease ContaminationProtein PurificationRecombinant Prototype Foamy Virus IntegraseE. ColiSonicationUltracentrifugationFPLCNickel-charged ResinSDS-PAGEUV Absorbance

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