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Erkennung und Entfernung von Nuklease Kontamination während der Reinigung von rekombinanten Prototyp schaumig Virus Integrase
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
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Erkennung und Entfernung von Nuklease Kontamination während der Reinigung von rekombinanten Prototyp schaumig Virus Integrase

6,896 Views

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07:50 min

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December 08, 2017

DOI:

10.3791/56605-v

DOI:
10.3791/56605-v

•

07:50 min
December 08, 2017

•

4 Views
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1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Summary

Automatically generated

Rekombinante Prototyp schaumig Virus Integrase Protein ist oft mit einer bakteriellen Nuklease während Reinigung kontaminiert. Diese Methode Nuklease Kontamination identifiziert und entfernt die letzten Vorbereitungen des Enzyms.

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Cite this Article

Lopez Jr., M. A., Mackler, R. M., Altman, M. P., Yoder, K. E. Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase. J. Vis. Exp. (130), e56605, doi:10.3791/56605 (2017).

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