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視床皮質軸索分岐とシナプスの可視化脊髄共培養系における形成

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Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures

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視床皮質軸索分岐とシナプスの可視化脊髄共培養系における形成

6,353 Views

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06:16 min

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March 28, 2018

DOI:

10.3791/56553-v

DOI:

10.3791/56553-v

•

06:16 min

March 28, 2018

•

1 Views

Naoyuki Matsumoto^1,2, Nobuhiko Yamamoto²

¹Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University, ²Neuroscience Laboratories, Graduate School of Frontier Biosciences,Osaka University

Summary

このプロトコルでは、視床皮質軸索分岐およびシナプス形成の脊髄共培養系における視床と大脳皮質の同時イメージング法について説明します。個々の視床皮質軸索とその神経終末は、下流と GFP タグシナプトフィジン単細胞エレクトロポレーション法による可視化します。

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