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Journal / Neuroscience / 用反褶积显微镜观察小鼠原发性下丘脑神经元 GLUT4…
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用反褶积显微镜观察小鼠原发性下丘脑神经元 GLUT4 蛋白的动态图像
 
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用反褶积显微镜观察小鼠原发性下丘脑神经元 GLUT4 蛋白的动态图像

Article DOI: 10.3791/56409-v • 08:47 min • December 7th, 2017
December 7th, 2017 •
1,2,3, 4,5, 4,5,6
1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine, Taipei Medical University, 3Core Facility, Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

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本协议描述了一种实时绿色荧光蛋白 (GFP) 标记葡萄糖转运蛋白 4 (GLUT4) 蛋白质贩运的技术, 对胰岛素刺激和 CCR5 在 insulin–GLUT4 的生物学作用的表征带反褶积显微镜的信号通路。

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神经生物学 问题 130 小鼠下丘脑神经元培养 活图像记录 脂质体的转染 GLUT4 反褶积显微镜
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