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Een alternatieve cultuur methode om Genomic Hypomethylation van muis embryonale stamcellen met MEK-remmer PD0325901 en vitamine C
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An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
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Een alternatieve cultuur methode om Genomic Hypomethylation van muis embryonale stamcellen met MEK-remmer PD0325901 en vitamine C

6,515 Views

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11:53 min

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June 01, 2018

DOI:

10.3791/56391-v

DOI:
10.3791/56391-v

•

11:53 min
June 01, 2018

•

1 Views
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1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences

Summary

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Wij beschreven twee chemische-gebaseerde protocollen voor het kweken van muis embryonale stamcellen in detail. Deze nieuwe methode maakt gebruik van synergetische mechanismen ter bevordering van Tet-gemedieerde oxidatie (door vitamine C) en onderdrukken van DOVO synthese van 5-methylcytosine (door PD0325901) om DNA hypomethylation en pluripotent van muis embryonale stamcellen te onderhouden.

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Mouse Embryonic Stem CellsGenomic HypomethylationMEK Inhibitor PD0325901Vitamin CCulture MethodDNA ExtractionDNA MethylationPluripotencyHeterogeneityMorphologyUndifferentiated State

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Li, C., Lai, W., Wang, H. An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C. J. Vis. Exp. (136), e56391, doi:10.3791/56391 (2018).

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