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An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
JoVE Journal
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JoVE Journal Biyokimya
An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

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11:53 min

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June 01, 2018

DOI:

10.3791/56391-v

DOI:
10.3791/56391-v

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11:53 min
June 01, 2018

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1 Views
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1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences

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We described in detail two chemical-based protocols for culturing mouse embryonic stem cells. This new method utilizes synergistic mechanisms of promoting Tet-mediated oxidation (by vitamin C) and repressing de novo synthesis of 5-methylcytosine (by PD0325901) to maintain DNA hypomethylation and pluripotency of mouse embryonic stem cells.

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Mouse Embryonic Stem CellsGenomic HypomethylationMEK Inhibitor PD0325901Vitamin CCulture MethodDNA ExtractionDNA MethylationPluripotencyHeterogeneityMorphologyUndifferentiated State

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Li, C., Lai, W., Wang, H. An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C. J. Vis. Exp. (136), e56391, doi:10.3791/56391 (2018).

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