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One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
JoVE 신문
암 연구학
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JoVE 신문 암 연구학
One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy

One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy

DOI:
10.3791/56338-v

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06:47 min

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October 23, 2017

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, , ,
1Department of Radiation Oncology,Gunma University Graduate School of Medicine, 2Advanced Scientific Research Leaders Development Unit,Gunma University Graduate School of Medicine

Chapters

  • 00:05Title
  • 01:31Cell Culture and Irradiation
  • 02:53Fixation and DAPI Staining
  • 04:44Results: Nuclear Morpholgy in Different Forms of Cell Death
  • 05:51Conclusion

Summary

자동 번역

자동 번역

Research on ionizing radiation (IR)-induced clonogenic cell death is important for understanding the effects of IR on malignant tumors and normal tissues. Here, we describe a one-step assay for assessing the major modes of IR-induced clonogenic cell death based on morphology of 4',6-diamidino-2-phenylindole dihydrochloride (DAPI)-stained nuclei visualized by fluorescence microscopy.

Tags

Radiation-induced Clonogenic Cell DeathFluorescence MicroscopyDAPI StainingCell Death ModesRadiation BiologyCell CultureX-ray IrradiationFixationCell AttachmentCell Suspension

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