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Cancer Research

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JoVE Journal Cancer Research
One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
 

One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy

Article DOI: 10.3791/56338-v • 06:47 min • October 23rd, 2017
October 23rd, 2017 •
1, 2, 1, 1
1Department of Radiation Oncology, Gunma University Graduate School of Medicine, 2Advanced Scientific Research Leaders Development Unit, Gunma University Graduate School of Medicine

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Research on ionizing radiation (IR)-induced clonogenic cell death is important for understanding the effects of IR on malignant tumors and normal tissues. Here, we describe a one-step assay for assessing the major modes of IR-induced clonogenic cell death based on morphology of 4',6-diamidino-2-phenylindole dihydrochloride (DAPI)-stained nuclei visualized by fluorescence microscopy.

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Keywords: Radiation-induced Clonogenic Cell Death Fluorescence Microscopy DAPI Staining Cell Death Modes Radiation Biology Cell Culture X-ray Irradiation Fixation Cell Attachment Cell Suspension
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