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Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
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October 23, 2017
DOI:
1Department of Translational Research,Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research),Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences,Tokyo Medical and Dental University
Summary
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We describe a facile, rapid, and relatively inexpensive method for the generation of gene-disrupted Streptococcus mutans strains; this technique may be adapted for the generation of gene-disrupted strains of various species.
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Murata, T., Okada, A., Matin, K., Hanada, N. Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning. J. Vis. Exp. (128), e56319, doi:10.3791/56319 (2017).
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