Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Immunostaining para modificaciones de ADN:…
JoVE Journal
Genetics

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Genetics
Immunostaining para modificaciones de ADN
 
Click here for the English version

Immunostaining para modificaciones de ADN: análisis computacional de imágenes confocales

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

챕터

요약
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Recién descubierto formas oxidadas de la 5-METILCITOSINA (oxi-mCs), 5-hidroximetilcitosina (5hmC), 5-formylcytosine (fC 5) y 5-carboxylcytosine (5caC) puede representar distintas modificaciones de ADN con papeles funcionales únicas. Aquí se describe un flujo de trabajo semi cuantitativa para la visualización de la distribución espacial de la oxi-mCs, perfiles de intensidad de señal y colocalización.

Tags

Genética número 127 epigenética ADN oxi-mCs N6-methyladenine Immunohistochemistry Zen microscopia Confocal señal de cuantificación distribución espacial
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter