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Genetics

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Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
 

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Article DOI: 10.3791/56313-v 09:08 min November 13th, 2017
November 13th, 2017

챕터

요약

Assay for Transposase-Accessible Chromatin coupled with high-throughput sequencing (ATAC-seq) is a genome-wide method to uncover accessible chromatin. This is a step-by-step ATAC-seq protocol, from molecular to the final computational analysis, optimized for human lymphocytes (Th1/Th2). This protocol can be adopted by researchers without prior experience in next-generation sequencing methods.

Tags

ATAC-Seq Accessible Chromatin Human T Lymphocytes Transposase Epigenomic Fields Regulatory Elements Chromatin Accessibility DNase-seq FAIRE-seq PBMCs RPMI Medium Culture Flask Incubate CD4 Positive Cells Microbead Separation Column
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